01 Taco Misfire

Hello all. Im new to posting here. I have had a reocurring problem with my 01 taco. Here is a little back ground before i get to the problem. About 3 weeks ago i found out i had a burnt exhaust valve and had to replace the motor since a valve job on a used motor wasnt an option with the miles i had on it. So after a new rebuilt motor and trans installed ive been getting a misfire in Cylinder 2. I have moved plugs, coil packs around to see if this causes the mis to move and it does not. I need some help on what else to check. During the engine rebuild i had my injectors checked all were within manufactures specs. I put all new plugs in during the rebuild and have replaced 2 coil packs. I need to get this thing running before fathers day next weekend. Any help is greatly appricated.